Fig1: Immunocytochemical staining of MCF-7 cells using anti-MYLK rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of SW480 cells using anti-MYLK rabbit polyclonal antibody.
Fig3: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-MYLK rabbit polyclonal antibody.
Fig4: Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MYLK rabbit polyclonal antibody.
Fig5: Flow cytometric analysis of MCF-7 cells with MYLK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.
Fig1: Immunocytochemical staining of MCF-7 cells using anti-MYLK rabbit polyclonal antibody.
Application
Fig2: Immunocytochemical staining of SW480 cells using anti-MYLK rabbit polyclonal antibody.
Application
Fig3: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-MYLK rabbit polyclonal antibody.
Application
Fig4: Immunohistochemical analysis of paraffin- embedded mouse smooth muscle tissue using anti-MYLK rabbit polyclonal antibody.
Application
Fig5: Flow cytometric analysis of MCF-7 cells with MYLK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
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