All products Antibodies Metadherin(LYRIC)

Metadherin(LYRIC)

Catalog Number：OM638886

  • Fig1: Western blot analysis of Metadherin(LYRIC) on human skeletal muscle tissue (1) and MCF-7 cell (2) lysate using anti-Metadherin(LYRIC) antibody at 1/1,000 dilution.

  • Fig2: ICC staining Metadherin(LYRIC) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining Metadherin(LYRIC) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining Metadherin(LYRIC) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of SH-SY5Y cells with Metadherin(LYRIC) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name Metadherin(LYRIC)
Antibody Type Primary Antibodies
Product description 
MTDH (metastasis adhesion protein), also known as astrocyte elevated gene-1 protein (AEG1) or lysine-rich CEACAM1 co-isolated protein (LYRIC), is a 582 amino acid single-pass membrane protein. Overexpressed in various cancers, MTDH activates the transcription factor NFκB and promotes anchorage-independent growth of immortalized astrocytes and melanocytes, which are key components of tumor growth. MTDH is also thought to enhance the seeding of tumor cells to the target organ endothelium. Localized mainly to the endoplasmic reticulum membrane, MTDH also translocates from the cytoplasm to the nucleus when induced by TNFα. MTDH also localizes to tight junctions (TJ) during the maturation of TJ complexes in epithelial cells. MTDH is found at highest levels in heart, skeletal muscle, tongue and small intestine.
Immunogen 
Recombinant protein

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:100-1:500
FC:1:50-1:200
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
3D3 antibody 3D3/LYRIC antibody AEG 1 antibody AEG-1 antibody AEG1 antibody Astrocyte elevated gene 1 antibody Astrocyte elevated gene-1 protein antibody LYRIC antibody LYRIC/3D3 antibody LYRIC_HUMAN antibody Lysine rich CEACAM1 associated protein antibody Lysine rich CEACAM1 co isolated protein antibody Lysine-rich CEACAM1 co-isolated protein antibody Metadherin antibody Metastasis adhesion protein antibody MTDH antibody Protein LYRIC antibody
Molecular Weight(MW) 64 kDa
Cellular Localization Endoplasmic reticulum membrane. Nucleus.

Database Links

SwissProt ID Q86UE4

Application

  • Application

    Fig1: Western blot analysis of Metadherin(LYRIC) on human skeletal muscle tissue (1) and MCF-7 cell (2) lysate using anti-Metadherin(LYRIC) antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining Metadherin(LYRIC) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Metadherin(LYRIC) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining Metadherin(LYRIC) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of SH-SY5Y cells with Metadherin(LYRIC) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Human skeletal muscle tissue lysate, MCF-7, Hela, N2A, SH-SY5Y, human breast tissue, mouse brain tissue.
Application Notes
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:100-1:500
FC:1:50-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638886
  • Price: $336.00
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  • Lead time: 4~6 Week

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