All products Antibodies MyoGEF Antibody (N-term)

MyoGEF Antibody (N-term)

Catalog Number：OM214910

  • Western blot analysis of MyoGEF Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Profile

Product Name MyoGEF Antibody (N-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Alternative Names
PLEKHG6
Pleckstrin homology domain-containing family G member 6
Myosin-interacting guanine nucleotide exchange factor
Molecular Weight(MW) 88960 Da
Function
Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis
Tissue Specificity Highest expression in the placenta. Low levels in small intestine, lung, liver, kidney, thymus and heart
Cellular Localization Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cleavage furrow Note=During mitosis, localizes to the spindle pole, central spindle and cleavage furrow. In epithelial cells, recruited to the apical membrane by EZR

Database Links

Entrez Gene 55200

Application

  • Application

    Western blot analysis of MyoGEF Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.

  • Application

    Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Submit an review for OM214910
Submit an review for OM214910
  • Catalog Number: OM214910
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: In stock
  • Lead time: 2~3 Week

  • Quantity:
    - +
  • Shipping to (Please select)
Add to cart
Add to Wishlist
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.
PRODUCT CATEGORIES
Primary antibodies Secondary antibodies Tags antibodies Flow cytometry antibodies
SUPPORT
Catalogs Newsletter Order Online Buying FAQs Distributor
COMPANY
Profile Strategy Careers
CONTACT
Contact us

2013 © Omnimabs , All Rights Reserved.