All products Antibodies NBN Antibody (C-term)

NBN Antibody (C-term)

Catalog Number：OM215020

  • NBN Antibody (C-term) western blot analysis in NCI-H460,NCI-H292 cell line lysates (35ug/lane).This demonstrates the NBN antibody detected the NBN protein (arrow).

  • Confocal immunofluorescent analysis of NBN Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • NBN Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Profile

Product Name NBN Antibody (C-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIFFC
WB~~1:100~500 IF~~1:10~50 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
NBS
NBS1
P95
Alternative Names
NBN
NBS
NBS1
P95
Nibrin
Cell cycle regulatory protein p95
Nijmegen breakage syndrome protein 1
Molecular Weight(MW) 84959 Da
Function
Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection
Tissue Specificity Ubiquitous. Expressed at high levels in testis
Cellular Localization Nucleus (By similarity). Chromosome, telomere (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity)

Database Links

Entrez Gene 4683
Nucleotide Accession NP_002476.2

Application

  • Application

    NBN Antibody (C-term) western blot analysis in NCI-H460,NCI-H292 cell line lysates (35ug/lane).This demonstrates the NBN antibody detected the NBN protein (arrow).

  • Application

    Confocal immunofluorescent analysis of NBN Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Application

    NBN Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IF~~1:10~50 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM215020
  • Price: $336.00
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  • Lead time: 2~3 Week

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