Western blot analysis of NCF4 antibody (C-term) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NCF4 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | NCF4 Antibody (C-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Gene Synonyms | SH3PXD4 |
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Alternative Names | NCF4 SH3PXD4 Neutrophil cytosol factor 4 Neutrophil NADPH oxidase factor 4 SH3 and PX domain-containing protein 4 p40-phox |
Molecular Weight(MW) | 39032 Da |
Function | Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex |
Tissue Specificity | Expression is restricted to hematopoietic cells |
Cellular Localization | Cytoplasm. |
Entrez Gene | 4689 |
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Western blot analysis of NCF4 antibody (C-term) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NCF4 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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