Western blot analysis of ND5 Antibody (C-term) in CEM cell line lysates (35ug/lane). ND5 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of CEM cells using ND5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | ND5 Antibody (C-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Gene Synonyms | MTND5 NADH5 ND5 |
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Alternative Names | MT-ND5 MTND5 NADH5 ND5 NADH-ubiquinone oxidoreductase chain 5 NADH dehydrogenase subunit 5 |
Molecular Weight(MW) | 67027 Da |
Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity) |
Tissue Specificity | This ND5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 550~579 amino acids from the C-terminal region of human ND5. |
Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein (By similarity) |
Entrez Gene | 4540 |
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Western blot analysis of ND5 Antibody (C-term) in CEM cell line lysates (35ug/lane). ND5 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of CEM cells using ND5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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