Western blot analysis of NDUFB4 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). NDUFB4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue with NDUFB4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of 293 cells using NDUFB4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | NDUFB4 Antibody (C-term) |
---|---|
Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
---|---|
Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Alternative Names | NDUFB4 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Complex I-B15 NADH-ubiquinone oxidoreductase B15 subunit |
---|---|
Molecular Weight(MW) | 15209 Da |
Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone |
Tissue Specificity | This NDUFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~127 amino acids from the C-terminal region of human NDUFB4. |
Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side |
Entrez Gene | 4710 |
---|
Western blot analysis of NDUFB4 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). NDUFB4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue with NDUFB4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of 293 cells using NDUFB4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
---|
Form | Liquid |
---|---|
Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
2013 © Omnimabs , All Rights Reserved.