Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.
NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | NEDD4 Antibody (C-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Gene Synonyms | KIAA0093 NEDD4-1 |
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Alternative Names | NEDD4 KIAA0093 NEDD4-1 E3 ubiquitin-protein ligase NEDD4 Cell proliferation-inducing gene 53 protein Neural precursor cell expressed developmentally down-regulated protein 4 |
Molecular Weight(MW) | 149114 Da |
Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates Involved in the pathway leading to the degradation of VEGFR- 2/KDFR, independently of its ubiquitin-ligase activity Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 |
Tissue Specificity | This NEDD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 977~1007 amino acids from the C-terminal region of human NEDD4. |
Cellular Localization | Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Note=Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1 |
Entrez Gene | 4734 |
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Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.
NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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