All products Antibodies NFKBIA Antibody ( S32/36 )

NFKBIA Antibody ( S32/36 )

Catalog Number：OM215295

  • Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Confocal immunofluorescent analysis of NFKBIA Antibody ( S32/36 )(Cat#AP7981b) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Product Profile

Product Name NFKBIA Antibody ( S32/36 )
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFCIF
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
IKBA
MAD3
NFKBI
Alternative Names
NFKBIA
IKBA
MAD3
NFKBI
NF-kappa-B inhibitor alpha
I-kappa-B-alpha
Major histocompatibility complex enhancer-binding protein MAD3
Molecular Weight(MW) 35609 Da
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription
Tissue Specificity This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~49 amino acids from human NFKBIA.
Cellular Localization Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity)

Database Links

Entrez Gene 4792

Application

  • Application

    Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.

  • Application

    Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Confocal immunofluorescent analysis of NFKBIA Antibody ( S32/36 )(Cat#AP7981b) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50 IF~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM215295
  • Price: $336.00
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  • Lead time: 2~3 Week

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