Western blot analysis of NFYA antibody (C-term) in Jurkat cell line lysates (35ug/lane). NFYA(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with NFYA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NFYA Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | NFYA Antibody (C-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Alternative Names | NFYA Nuclear transcription factor Y subunit alpha CAAT box DNA-binding protein subunit A Nuclear transcription factor Y subunit A |
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Molecular Weight(MW) | 36877 Da |
Function | Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes |
Tissue Specificity | This NFYA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284~313 amino acids from the C-terminal region of human NFYA. |
Cellular Localization | Nucleus. |
Entrez Gene | 4800 |
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Western blot analysis of NFYA antibody (C-term) in Jurkat cell line lysates (35ug/lane). NFYA(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with NFYA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NFYA Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
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