All products Antibodies NIX Antibody (Center)

NIX Antibody (Center)

Catalog Number：OM215370

  • NIX Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).

  • NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

  • Confocal immunofluorescent analysis of NIX Antibody (Center)(Cat#AP11786c) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Profile

Product Name NIX Antibody (Center)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCIFFC
WB~~1:100~500 IHC~~1:50~100 IF~~1:10~50 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
BNIP3A
BNIP3H
NIX
Alternative Names
BNIP3L
BNIP3A
BNIP3H
NIX
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
Adenovirus E1B19K-binding protein B5
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
NIP3-like protein X
Molecular Weight(MW) 23930 Da
Function
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor
Tissue Specificity This NIX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the Central region of human NIX.
Cellular Localization Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane; Single-pass membrane protein (Potential). Note=Colocalizes with SPATA18 at the mitochondrion outer membrane

Database Links

Entrez Gene 665
Nucleotide Accession NP_004322.1

Application

  • Application

    NIX Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).

  • Application

    NIX Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

  • Application

    Confocal immunofluorescent analysis of NIX Antibody (Center)(Cat#AP11786c) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Application

    NIX Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:50~100 IF~~1:10~50 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM215370
  • Price: $336.00
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  • Lead time: 2~3 Week

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