NM23

Catalog Number：OM638939

  • Fig1: Western blot analysis of NM23 on Hela (1) and HepG2 (2) cell lysate using anti-NM23 antibody at 1/1,000 dilution.

  • Fig2: ICC staining NM23 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining NM23 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4:  Flow cytometric analysis of Jurkat cells with NM23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Profile

Product Name NM23
Antibody Type Primary Antibodies
Product description 
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease-hypersensitive elements at positions 142 to 115 of the human C-Myc promotor.
Immunogen 
Peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
AWD antibody AWD
drosophila
homolog of antibody GAAD antibody Granzyme A activated DNase antibody Granzyme A-activated DNase antibody GZMA activated DNase antibody Metastasis inhibition factor NM23 antibody NB antibody NBS antibody NDK A antibody NDKA antibody NDKA_HUMAN antibody NDP kinase A antibody NDPK-A antibody NDPKA antibody NM23 antibody NM23 long variant
included antibody nm23-H1 antibody NM23-M1 antibody NM23H1B
included antibody NME/NM23 nucleoside diphosphate kinase 1 antibody Nme1 antibody NME1-NME2 spliced read-through transcript
included antibody Non-metastatic cells 1
protein (NM23A) expressed in antibody Nonmetastatic cells 1
protein expressed in antibody Nonmetastatic protein 23 antibody Nonmetastatic protein 23
homolog 1 antibody Nucleoside diphosphate kinase A antibody Tumor metastatic process-associated protein antibody
Molecular Weight(MW) 17/19 kDa
Cellular Localization Cytoplasm. Nucleus.

Database Links

SwissProt ID P15531
P15532

Application

  • Application

    Fig1: Western blot analysis of NM23 on Hela (1) and HepG2 (2) cell lysate using anti-NM23 antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining NM23 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining NM23 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4:  Flow cytometric analysis of Jurkat cells with NM23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Positive Control Hela, HepG2, MCF-7, Jurkat.
Application Notes
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638939
  • Price: $336.00
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