Western blot analysis of NMI Antibody (N-term) in Hela cell line lysates (35ug/lane). NMI (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NMI Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clini relevance has not been evaluated.
NMI Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Name | NMI Antibody (N-term) |
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Antibody Type | Primary Antibodies |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | |
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Alternative Names | NMI N-myc-interactor N-myc and STAT interactor |
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Molecular Weight(MW) | 35057 Da |
Function | May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5 |
Tissue Specificity | Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver |
Cellular Localization | Cytoplasm. |
Entrez Gene | 9111 |
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Western blot analysis of NMI Antibody (N-term) in Hela cell line lysates (35ug/lane). NMI (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NMI Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clini relevance has not been evaluated.
NMI Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50: |
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Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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