NOL9 Antibody (C-term) western blot analysis in Jurkat,293,Hela cell line lysates (35ug/lane).This demonstrates the NOL9 antibody detected the NOL9 protein (arrow).
NOL9 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NOL9 Polynucleotide 5'-hydroxyl-kinase NOL9 Nucleolar protein 9
Molecular Weight(MW)
79323 Da
Function
Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA
Tissue Specificity
This NOL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 673~702 amino acids from the C-terminal region of human NOL9.
Cellular Localization
Nucleus (By similarity). Nucleus, nucleolus. Note=Colocalizes with pre-60S rRNP particles
NOL9 Antibody (C-term) western blot analysis in Jurkat,293,Hela cell line lysates (35ug/lane).This demonstrates the NOL9 antibody detected the NOL9 protein (arrow).
Application
NOL9 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes
WB~~1:100~500 FC~~1:10~50:
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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