All products Antibodies Nanos Homologue 1 (NANOS1) Antibody (C-term)

Nanos Homologue 1 (NANOS1) Antibody (C-term)

Catalog Number：OM214983

  • Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab (1:60 dilution).

  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

  • Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Profile

Product Name Nanos Homologue 1 (NANOS1) Antibody (C-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCIFFC
WB~~1:50~100 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Gene Synonyms
NOS1
Alternative Names
NANOS1
NOS1
Nanos homolog 1
EC_Rep1a
Molecular Weight(MW) 30230 Da
Function
May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin Up-regulates the production of MMP14 to promote tumor cell invasion
Tissue Specificity Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness
Cellular Localization Cytoplasm, perinuclear region. Cytoplasm. Note=Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells

Database Links

Entrez Gene 340719

Application

  • Application

    Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab (1:60 dilution).

  • Application

    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

  • Application

    Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:50~100 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Submit an review for OM214983
Submit an review for OM214983
  • Catalog Number: OM214983
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: In stock
  • Lead time: 2~3 Week

  • Quantity:
    - +
  • Shipping to (Please select)
Add to cart
Add to Wishlist
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.
PRODUCT CATEGORIES
Primary antibodies Secondary antibodies Tags antibodies Flow cytometry antibodies
SUPPORT
Catalogs Newsletter Order Online Buying FAQs Distributor
COMPANY
Profile Strategy Careers
CONTACT
Contact us

2013 © Omnimabs , All Rights Reserved.