All products Antibodies Neuron-specific class Ⅲ β-tubulin

Neuron-specific class Ⅲ β-tubulin

Catalog Number：OM626608

  • Fig1: Western blot analysis of Tubulin beta-3 chain on Hela cell lysate using anti-Tubulin beta-3 chain antibody at 1/10,000 dilution.

  • Fig2: ICC staining Tubulin beta-3 chain in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining Tubulin beta-3 chain in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of Hela cells with Tubulin beta-3 chain antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Profile

Product Name Neuron-specific class Ⅲ β-tubulin
Antibody Type Primary Antibodies
Product description 
Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.
Immunogen 
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of tubulin beta-3.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:5,000-1:10,000
ICC:1:50-1:200
IHC:1:100-1:400
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
beta 3 tubulin antibody beta-4 antibody CDCBM antibody CDCBM1 antibody CFEOM3 antibody CFEOM3A antibody FEOM3 antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tubulin antibody Neuron-specific class III beta-tubulin antibody QccE-11995 antibody QccE-15186 antibody TBB3_HUMAN antibody Tubb 3 antibody TUBB3 antibody TUBB4 antibody Tubulin beta 3 antibody Tubulin beta 3 chain antibody Tubulin beta 4 antibody Tubulin beta III antibody Tubulin beta-3 chain antibody Tubulin beta-4 chain antibody Tubulin beta-III antibody
Molecular Weight(MW) 50 kDa
Cellular Localization Cytoplasm. Cytoskeleton. Microtubule.

Database Links

SwissProt ID Q13509
Q9ERD7
Q4QRB4

Application

  • Application

    Fig1: Western blot analysis of Tubulin beta-3 chain on Hela cell lysate using anti-Tubulin beta-3 chain antibody at 1/10,000 dilution.

  • Application

    Fig2: ICC staining Tubulin beta-3 chain in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Tubulin beta-3 chain in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of Hela cells with Tubulin beta-3 chain antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control Hela, HepG2, NIH-3T3, rat brain tissue, rat kidney tissue, human colon cancer tissue.
Application Notes
WB:1:5,000-1:10,000
ICC:1:50-1:200
IHC:1:100-1:400
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626608
  • Price: $210.00
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