All products Antibodies Rabbit anti-NOTCH3) Antibody

Rabbit anti-NOTCH3) Antibody

Catalog Number：OM105612

  • WB Suggested Anti-PCGF3 Antibody Titration: 0.125ug/ml
    ELISA Titer: 1:12500
    Positive Control: Raji cell lysate

    PCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells

  • Host: Rabbit
    Target Name: PCGF3
    Sample Type: Fetal Muscle lysates
    Antibody Dilution: 1.0ug/ml

  • Host: Rabbit
    Target Name: F13B
    Sample Tissue: Human DLD1
    Antibody Dilution: 1.0ug/ml

  • Rabbit Anti-F13B antibody
    Catalog Number: ARP41999
    Formalin Fixed Paraffin Embedded Tissue: Human Fetal Liver
    Primary antibody Concentration: 1:100
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody Concentration: 1:200
    Magnification: 20x
    Exposure Time: 0.5-2.0sec

  • Rabbit Anti-F13B antibody
    Catalog Number: ARP41999
    Formalin Fixed Paraffin Embedded Tissue: Human Vessel
    Primary antibody Concentration: 1:100
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody Concentration: 1:200
    Magnification: 20x
    Exposure Time: 0.5-2.0sec

  • WB Suggested Anti-FBXO25 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Human heart

  • Host: Rabbit
    Target Name: MARCH5
    Sample Type: U937 Whole Cell lysates
    Antibody Dilution: 1.0ug/ml

  • WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:1562500
    Positive Control: 721_B cell lysate

  • WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/ml
    Positive Control: HepG2 cell lysate

  • WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
    Positive Control: Human heart

  • WB Suggested Anti-ZNF599 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: 293T cell lysate

  • WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: Human kidney

  • WB Suggested Anti-UBQLN1 Antibody Titration: 1 ug/ml
    Positive Control: 293T cells lysate

  • Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).

  • Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.

  • WB Suggested Anti-Vwa5a Antibody
    Titration: 1.0 ug/ml
    Positive Control: Mouse Kidney

  • Host: Rabbit
    Target Name: SPATA2
    Sample Type: HCT15 Whole Cell lysates
    Antibody Dilution: 1.0ug/ml

  • WB Suggested Anti-ADAM8 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: PANC1 cell lysate

  • WB Suggested Anti-ORMDL3 Antibody
    Titration: 1.0 ug/ml
    Positive Control: Jurkat Whole Cell

  • Host: Rabbit
    Target Name: NR4A1
    Sample Tissue: Human Hela
    Antibody Dilution: 1.0ug/ml

  • Rabbit Anti-NR4A1 Antibody
    Catalog Number: ARP31941
    Paraffin Embedded Tissue: Human Stomach
    Cellular Data: Mucous Cells
    Antibody Concentration: 4.0-8.0 ug/ml
    Magnification: 400X

  • Rabbit Anti-NR4A1 Antibody
    Catalog Number: ARP31941
    Paraffin Embedded Tissue: Human Liver
    Cellular Data: Hepatocytes
    Antibody Concentration: 4.0-8.0 ug/ml
    Magnification: 400X

  • Lanes:
    Lane 1: 50ug mouse embryonic fibroblast lysate
    Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate
    Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Donkey anti-Rabbit-HRP
    Secondary Antibody Dilution:
    1:10000
    Gene Name:
    PTRF
    Submitted by:
    Libin Liu, Boston University.

  • Host: Rabbit
    Target Name: PTRF
    Sample Tissue: Mouse Lung
    Antibody Dilution: 1.0ug/ml

  • IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.

  • Lanes:
    Lane 1: 5 ug 293T cell lysate
    Lane 2: 10 ug 293T cell lysate
    Lane 3: 20 ug 293T cell lysate
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Anti-rabbit HRP
    Secondary Antibody Dilution:
    1:10000
    Gene Name:
    HMGA2
    Submitted by:
    Anonymous

    HMGA2 is supported by BioGPS gene expression data to be expressed in HEK293T

  • WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/ml
    ELISA Titer: 1:62500
    Positive Control: Jurkat cell lysate

  • PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.

    There is BioGPS gene expression data showing that PKLR is expressed in HepG2

  • Rabbit Anti-PKLR Antibody
    Catalog Number: ARP41699
    Paraffin Embedded Tissue: Human Kidney
    Cellular Data: Epithelial cells of renal tubule
    Antibody Concentration: 4.0-8.0 ug/ml
    Magnification: 400X

  • Sample Type: Equine Cartilage Explants
    Primary Dilution: 1:800
    Secondary Antibody: Bio-Rad 170-5046
    Secondary Dilution::100,000
    Image Submitted By: Adam Williams
    University of Nottingham

  • WB Suggested Anti-MMP1 Antibody
    Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti-rabbit-HRP
    Secondry Antibody Dilution : 1:2000
    Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore

  • Host: Rabbit
    Target Name: MMP1
    Sample Tissue: Human 293T
    Antibody Dilution: 1.0ug/ml

  • Host: Rabbit
    Target Name: MARCH5
    Sample Type: Human Fetal Kidney
    Antibody Dilution: 1.0ug/ml

  • Host: Rabbit
    Target Name: G6pc
    Sample Tissue: Mouse Lung
    Antibody Dilution: 1.0ug/ml

  • Host: Rabbit
    Target Name: G6PC
    Sample Tissue: Mouse Testis
    Antibody Dilution: 1ug/ml

  • Host: Rabbit
    Target Name: SPPL2B
    Sample Type: 293T Whole cell lysates
    Antibody Dilution: 1.0ug/ml

  • WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Human Spleen

  • WB Suggested Anti-NUP35 Antibody Titration: 0.2-1 ug/ml
    Positive Control: Jurkat cell lysate

  • WB Suggested Anti-Amigo2 Antibody
    Titration: 1.0 ug/ml
    Positive Control: Rat Heart

  • WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: 721_B cell lysate

    ACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

  • ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.

  • WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:12500
    Positive Control: Human Liver

  • WB Suggested Anti-GLIS2 Antibody Titration: 2.5ug/ml
    ELISA Titer: 1:12500
    Positive Control: HepG2 cell lysate

  • WB Suggested Anti-RBM14 Antibody Titration: 1.25ug/ml
    ELISA Titer: 1:312500
    Positive Control: SP2/0 cell lysate

  • Lanes:
    Lane 1: 50ug Hela Lysate
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Anti-rabbit-HRP
    Secondary Antibody Dilution:
    1:10,000
    Gene Name:
    RBM14
    Submitted by:
    Archa Fox, University of Western Australia

Product Profile

Product Name Rabbit anti-NOTCH3) Antibody
Antibody Type Primary Antibodies
Immunogen 
Synthetic peptide directed towards the C terminal of human NOTCH3

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

BovineHumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol NOTCH3
Gene Synonyms
PCGF3
Gene Full Name Notch 3
Gene Summary
NOTCH3 encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Alternative Names
RNF3
DONG1
RNF3A
Molecular Weight(MW) 244kDa
Sequence 2321 amino acids

Database Links

Entrez Gene 4854
SwissProt ID Q9UM47
Protein Accession NP_000426

Application

  • Application

    WB Suggested Anti-PCGF3 Antibody Titration: 0.125ug/ml
    ELISA Titer: 1:12500
    Positive Control: Raji cell lysate

    PCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells

  • Application

    Host: Rabbit
    Target Name: PCGF3
    Sample Type: Fetal Muscle lysates
    Antibody Dilution: 1.0ug/ml

  • Application

    Host: Rabbit
    Target Name: F13B
    Sample Tissue: Human DLD1
    Antibody Dilution: 1.0ug/ml

  • Application

    Rabbit Anti-F13B antibody
    Catalog Number: ARP41999
    Formalin Fixed Paraffin Embedded Tissue: Human Fetal Liver
    Primary antibody Concentration: 1:100
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody Concentration: 1:200
    Magnification: 20x
    Exposure Time: 0.5-2.0sec

  • Application

    Rabbit Anti-F13B antibody
    Catalog Number: ARP41999
    Formalin Fixed Paraffin Embedded Tissue: Human Vessel
    Primary antibody Concentration: 1:100
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody Concentration: 1:200
    Magnification: 20x
    Exposure Time: 0.5-2.0sec

  • Application

    WB Suggested Anti-FBXO25 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Human heart

  • Application

    Host: Rabbit
    Target Name: MARCH5
    Sample Type: U937 Whole Cell lysates
    Antibody Dilution: 1.0ug/ml

  • Application

    WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:1562500
    Positive Control: 721_B cell lysate

  • Application

    WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/ml
    Positive Control: HepG2 cell lysate

  • Application

    WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
    Positive Control: Human heart

  • Application

    WB Suggested Anti-ZNF599 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: 293T cell lysate

  • Application

    WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: Human kidney

  • Application

    WB Suggested Anti-UBQLN1 Antibody Titration: 1 ug/ml
    Positive Control: 293T cells lysate

  • Application

    Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).

  • Application

    Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.

  • Application

    WB Suggested Anti-Vwa5a Antibody
    Titration: 1.0 ug/ml
    Positive Control: Mouse Kidney

  • Application

    Host: Rabbit
    Target Name: SPATA2
    Sample Type: HCT15 Whole Cell lysates
    Antibody Dilution: 1.0ug/ml

  • Application

    WB Suggested Anti-ADAM8 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: PANC1 cell lysate

  • Application

    WB Suggested Anti-ORMDL3 Antibody
    Titration: 1.0 ug/ml
    Positive Control: Jurkat Whole Cell

  • Application

    Host: Rabbit
    Target Name: NR4A1
    Sample Tissue: Human Hela
    Antibody Dilution: 1.0ug/ml

  • Application

    Rabbit Anti-NR4A1 Antibody
    Catalog Number: ARP31941
    Paraffin Embedded Tissue: Human Stomach
    Cellular Data: Mucous Cells
    Antibody Concentration: 4.0-8.0 ug/ml
    Magnification: 400X

  • Application

    Rabbit Anti-NR4A1 Antibody
    Catalog Number: ARP31941
    Paraffin Embedded Tissue: Human Liver
    Cellular Data: Hepatocytes
    Antibody Concentration: 4.0-8.0 ug/ml
    Magnification: 400X

  • Application

    Lanes:
    Lane 1: 50ug mouse embryonic fibroblast lysate
    Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate
    Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Donkey anti-Rabbit-HRP
    Secondary Antibody Dilution:
    1:10000
    Gene Name:
    PTRF
    Submitted by:
    Libin Liu, Boston University.

  • Application

    Host: Rabbit
    Target Name: PTRF
    Sample Tissue: Mouse Lung
    Antibody Dilution: 1.0ug/ml

  • Application

    IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.

  • Application

    Lanes:
    Lane 1: 5 ug 293T cell lysate
    Lane 2: 10 ug 293T cell lysate
    Lane 3: 20 ug 293T cell lysate
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Anti-rabbit HRP
    Secondary Antibody Dilution:
    1:10000
    Gene Name:
    HMGA2
    Submitted by:
    Anonymous

    HMGA2 is supported by BioGPS gene expression data to be expressed in HEK293T

  • Application

    WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/ml
    ELISA Titer: 1:62500
    Positive Control: Jurkat cell lysate

  • Application

    PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.

    There is BioGPS gene expression data showing that PKLR is expressed in HepG2

  • Application

    Rabbit Anti-PKLR Antibody
    Catalog Number: ARP41699
    Paraffin Embedded Tissue: Human Kidney
    Cellular Data: Epithelial cells of renal tubule
    Antibody Concentration: 4.0-8.0 ug/ml
    Magnification: 400X

  • Application

    Sample Type: Equine Cartilage Explants
    Primary Dilution: 1:800
    Secondary Antibody: Bio-Rad 170-5046
    Secondary Dilution::100,000
    Image Submitted By: Adam Williams
    University of Nottingham

  • Application

    WB Suggested Anti-MMP1 Antibody
    Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti-rabbit-HRP
    Secondry Antibody Dilution : 1:2000
    Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore

  • Application

    Host: Rabbit
    Target Name: MMP1
    Sample Tissue: Human 293T
    Antibody Dilution: 1.0ug/ml

  • Application

    Host: Rabbit
    Target Name: MARCH5
    Sample Type: Human Fetal Kidney
    Antibody Dilution: 1.0ug/ml

  • Application

    Host: Rabbit
    Target Name: G6pc
    Sample Tissue: Mouse Lung
    Antibody Dilution: 1.0ug/ml

  • Application

    Host: Rabbit
    Target Name: G6PC
    Sample Tissue: Mouse Testis
    Antibody Dilution: 1ug/ml

  • Application

    Host: Rabbit
    Target Name: SPPL2B
    Sample Type: 293T Whole cell lysates
    Antibody Dilution: 1.0ug/ml

  • Application

    WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Human Spleen

  • Application

    WB Suggested Anti-NUP35 Antibody Titration: 0.2-1 ug/ml
    Positive Control: Jurkat cell lysate

  • Application

    WB Suggested Anti-Amigo2 Antibody
    Titration: 1.0 ug/ml
    Positive Control: Rat Heart

  • Application

    WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: 721_B cell lysate

    ACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

  • Application

    ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.

  • Application

    WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml
    ELISA Titer: 1:12500
    Positive Control: Human Liver

  • Application

    WB Suggested Anti-GLIS2 Antibody Titration: 2.5ug/ml
    ELISA Titer: 1:12500
    Positive Control: HepG2 cell lysate

  • Application

    WB Suggested Anti-RBM14 Antibody Titration: 1.25ug/ml
    ELISA Titer: 1:312500
    Positive Control: SP2/0 cell lysate

  • Application

    Lanes:
    Lane 1: 50ug Hela Lysate
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Anti-rabbit-HRP
    Secondary Antibody Dilution:
    1:10,000
    Gene Name:
    RBM14
    Submitted by:
    Archa Fox, University of Western Australia

Application Notes
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM105612
  • Price: $336.00
  • Discount Price
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  • Store: In stock
  • Lead time: 2~3 Week

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