All products Antibodies Rabbit anti-Onecut1 polyclonal antibody - C-terminal region

Rabbit anti-Onecut1 polyclonal antibody - C-terminal region

Catalog Number：OM105550

  • Immunohistochemistry with mouse embryo tissue tissue

  • Sample Type: Frozen section of mouse embryo at et12.5
    Primary Dilution: 1:500
    Secondary Dilution: 1:2000
    Antigen Retrevial method: Citrate buffer (pH=6) 
    Image Submitted By: Frédéric Clotman
    Université catholique de Louvain 

  • Onecut1 antibody - C-terminal region validated by WB using Mouse Liver lysate at 1.0ug/ml.

Product Profile

Product Name Rabbit anti-Onecut1 polyclonal antibody - C-terminal region
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIHC
WB:1:500~1:2000
IHC:1:50~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

BovineDogGuinea PigHumanMousePigRabbitRatZebra Fish
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol Onecut1
Gene Synonyms
D9Ertd423e
Hfh12
Hnf6
Hnf6b
OC-1
Oc1
HNF-6
Gene Full Name One cut domain, family member 1
Gene Summary
Onecut1 is a transcriptional activator. It binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. It is mportant for liver genes transcription and stimulates the expression of Onecut3 in the developing endoderm.
Alternative Names
D9Ertd423e
Hfh12
Hnf6
Hnf6b
OC-1
Oc1
HNF-6
Molecular Weight(MW) 51kDa
Sequence 465 amino acids

Database Links

Entrez Gene 15379
SwissProt ID O08755
Protein Accession NP_032288

Application

  • Immunohistochemistry

    Immunohistochemistry with mouse embryo tissue tissue

  • Immunohistochemical

    Sample Type: Frozen section of mouse embryo at et12.5
    Primary Dilution: 1:500
    Secondary Dilution: 1:2000
    Antigen Retrevial method: Citrate buffer (pH=6) 
    Image Submitted By: Frédéric Clotman
    Université catholique de Louvain 

  • Western blot

    Onecut1 antibody - C-terminal region validated by WB using Mouse Liver lysate at 1.0ug/ml.

Application Notes
WB:1:500~1:2000
IHC:1:50~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM105550
  • Price: $336.00
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  • Store: In stock
  • Lead time: 2~3 Week

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