All products Antibodies Rabbit anti-RAD17 polyclonal antibody - C-terminal region

Rabbit anti-RAD17 polyclonal antibody - C-terminal region

Catalog Number：OM105162

  • Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti-rabbit-HRP
    Secondry Antibody Dilution : 1:3000
    Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS

  • 1.25ug/ml
    ELISA Titer: 1:62500
    Positive Control: Jurkat cell lysate

Product Profile

Product Name Rabbit anti-RAD17 polyclonal antibody - C-terminal region
Antibody Type Primary Antibodies
Immunogen 
The immunogen for anti-RAD17 antibody: synthetic peptide directed towards the C terminal of human RAD17

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

BovineDogHumanMousePigRat
Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol RAD17
Gene Synonyms
CCYC
HRAD17
R24L
RAD17Sp
Rad24
RAD24
RAD17SP
Gene Full Name RAD17 homolog (S. pombe)
Gene Summary
RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.
Alternative Names
CCYC
HRAD17
R24L
RAD17Sp
Rad24
RAD24
RAD17SP
Molecular Weight(MW) 66kDa
Sequence 584 amino acids

Database Links

Entrez Gene 5884
SwissProt ID O75943-4
Protein Accession NP_579919

Application

  • Western blot

    Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti-rabbit-HRP
    Secondry Antibody Dilution : 1:3000
    Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS

  • Western blot

    1.25ug/ml
    ELISA Titer: 1:62500
    Positive Control: Jurkat cell lysate

Application Notes
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM105162
  • Price: $336.00
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  • Lead time: 2~3 Week

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