SIRT3

Catalog Number：OM638904

  • Fig1: Western blot analysis on different lysates using anti-SIRT3 rabbit polyclonal antibody. Positive control: Lane 1: A172 Lane 2: Mouse liver Lane 3: NIH/3T3 Lane 4: Mouse kidney Lane 5: F9

  • Fig2： Immunocytochemical staining of MCF-7 cells using anti-SIRT3 rabbit polyclonal antibody.

  • Fig3： Immunocytochemical staining of HepG2 cells using anti-SIRT3 rabbit polyclonal antibody.

  • Fig4： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-SIRT3 rabbit polyclonal antibody.

  • Fig5： Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-SIRT3 rabbit polyclonal antibody.

  • Fig6： Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Product Profile

Product Name SIRT3
Antibody Type Primary Antibodies
Product description 
The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.
Immunogen 
peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
hSIRT 3 antibody hSIRT3 antibody Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody NAD dependent deacetylase sirtuin 3 mitochondrial antibody NAD-dependent protein deacetylase sirtuin-3
mitochondrial antibody Regulatory protein SIR2 homolog 3 antibody Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody Sir 2 like 3 antibody SIR 2 like protein 3 antibody SIR 3 antibody SIR2 L3 antibody Sir2 like 3 antibody SIR2 like protein 3 antibody SIR2-like protein 3 antibody SIR2L3 antibody SIR3_HUMAN antibody SIRT 3 antibody SIRT3 antibody Sirtuin 3 antibody Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody Sirtuin type 3 antibody Sirtuin3 antibody
Molecular Weight(MW) 44/28kDa
Cellular Localization Mitochondrion matrix

Database Links

SwissProt ID Q9NTG7

Application

  • Application

    Fig1: Western blot analysis on different lysates using anti-SIRT3 rabbit polyclonal antibody. Positive control: Lane 1: A172 Lane 2: Mouse liver Lane 3: NIH/3T3 Lane 4: Mouse kidney Lane 5: F9

  • Application

    Fig2： Immunocytochemical staining of MCF-7 cells using anti-SIRT3 rabbit polyclonal antibody.

  • Application

    Fig3： Immunocytochemical staining of HepG2 cells using anti-SIRT3 rabbit polyclonal antibody.

  • Application

    Fig4： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-SIRT3 rabbit polyclonal antibody.

  • Application

    Fig5： Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-SIRT3 rabbit polyclonal antibody.

  • Application

    Fig6： Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Positive Control A172, NIH/3T3, MCF-7, HepG2, mouse kidney tissue, human kidney tissue
Application Notes
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638904
  • Price: $336.00
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