All products Antibodies Stat5 Antibody (C-17)

Stat5 Antibody (C-17)

Catalog Number：OM169105

  • Western blot analysis of Stat5b expression in SK-BR-3 (A) and K-562 (B) whole cell lysates.

  • Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).

  • Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).

  • Western blot analysis of Stat5 expression in 293T (A) and Jurkat (B) whole cell lysates.

  • Western blot analysis of Stat5b expression in non-transfected 293T: (A), human Stat5b transfected 293T: (B) and BJAB (C) whole cell lysates.

  • Western blot analysis of Stat5a expression in non-transfected 293T: (A), human Stat5a transfected 293T: (B) and K-562 (C) whole cell lysates.

  • Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5 (C-17), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Western blot analysis of Stat5 expression in Y79 (A), A-673 (B), K-562 (C) and Jurkat (D) nuclear extracts.

  • Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.

Product Profile

Product Name Stat5 Antibody (C-17)
Antibody Type Primary Antibodies
Modification Notes Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phos-phorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retin-oic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFFCELISA
recommended for detection of Stat5a and Stat5b of mouse, rat and human origin by WB, IP, IF, FCM, ELISA; also reactive with additional species, including equine, canine, bovine and porcine:
Species Reactivity

HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping at the C-terminus of Stat5 of mouse origin
Tissue Specificity epitope mapping at the C-terminus of Stat5 of mouse origin

Database Links

Entrez Gene 6776

Application

  • Application

    Western blot analysis of Stat5b expression in SK-BR-3 (A) and K-562 (B) whole cell lysates.

  • Application

    Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).

  • Application

    Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).

  • Application

    Western blot analysis of Stat5 expression in 293T (A) and Jurkat (B) whole cell lysates.

  • Application

    Western blot analysis of Stat5b expression in non-transfected 293T: (A), human Stat5b transfected 293T: (B) and BJAB (C) whole cell lysates.

  • Application

    Western blot analysis of Stat5a expression in non-transfected 293T: (A), human Stat5a transfected 293T: (B) and K-562 (C) whole cell lysates.

  • Application

    Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5 (C-17), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Western blot analysis of Stat5 expression in Y79 (A), A-673 (B), K-562 (C) and Jurkat (D) nuclear extracts.

  • Application

    Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.

Application Notes
recommended for detection of Stat5a and Stat5b of mouse, rat and human origin by WB, IP, IF, FCM, ELISA; also reactive with additional species, including equine, canine, bovine and porcine:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM169105
  • Price: $336.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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