All products Antibodies Synaptophysin

Synaptophysin

Catalog Number：OM626557

  • Fig1: Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.

  • Fig2: ICC staining Synaptophysin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

  • Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

  • Fig5: Flow cytometric analysis of SHG-44 cells with Synaptophysin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Profile

Product Name Synaptophysin
Antibody Type Primary Antibodies
Product description 
Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Immunogen 
Synthetic peptide (KLH-coupled) within human Synaptophysin 220-280 aa.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
Major synaptic vesicle protein p38 antibody MRX96 antibody MRXSYP antibody Syn p38 antibody Synaptophysin antibody Syp antibody SYPH antibody SYPH_HUMAN antibody SypI antibody
Molecular Weight(MW) 34kDa
Cellular Localization Cytoplasmic vesicle. Membrane. Synapse. Synaptosome.

Database Links

SwissProt ID P08247
Q62277
P07825

Application

  • Application

    Fig1: Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining Synaptophysin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Flow cytometric analysis of SHG-44 cells with Synaptophysin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control Human brain tissue, mouse brain tissue, N2A, rat pancreas tissue, SHG-44.
Application Notes
WB:1:500~1:2000
ICC:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626557
  • Price: $210.00
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  • Lead time: 4~6 Week

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