TGF-Beta 1

Catalog Number：OM626584

  • Fig1: Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control: Lane 1: Raji Lane 2: MCF-2 Lane 3: A549 Lane 4: Human kidney

  • Fig2: ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining TGF-Beta 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: ICC staining TGF-Beta 1 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Product Profile

Product Name TGF-Beta 1
Antibody Type Primary Antibodies
Product description 
Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly.
Immunogen 
peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:100:
Species Reactivity

HumanMouseRatZebra Fish
Concentration 1 mg/mL.

Target Information

Alternative Names
Cartilage-inducing factor antibody CED antibody Differentiation inhibiting factor antibody DPD1 antibody LAP antibody Latency-associated peptide antibody Prepro transforming growth factor beta 1 antibody TGF beta 1 antibody TGF beta antibody TGF beta 1 protein antibody TGF-beta 1 protein antibody TGF-beta-1 antibody TGF-beta-5 antibody TGF-beta1 antibody TGFB antibody Tgfb-1 antibody tgfb1 antibody TGFB1_HUMAN antibody TGFbeta antibody TGFbeta1 antibody Transforming Growth Factor b1 antibody Transforming Growth Factor beta 1 antibody Transforming growth factor beta 1a antibody transforming growth factor beta-1 antibody transforming growth factor
beta 1 antibody Transforming Growth Factor-?1 antibody
Molecular Weight(MW) 44 kDa
Cellular Localization Secreted

Database Links

SwissProt ID P01137

Application

  • Application

    Fig1: Western blot analysis of TGF-Beta 1 on different cell lysates using anti- TGF-Beta 1 antibody at 1/1000 dilution. Positive control: Lane 1: Raji Lane 2: MCF-2 Lane 3: A549 Lane 4: Human kidney

  • Application

    Fig2: ICC staining TGF-Beta 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining TGF-Beta 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: ICC staining TGF-Beta 1 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of HepG2 cells with TGF-Beta 1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Positive Control Raji, MCF-7, A549, human kidney tissue, mouse kidney tissue, SKBR-3, Hela, HepG2
Application Notes
WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:100:

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626584
  • Price: $386.00
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