Tyrosinase

Catalog Number：OM638963

  • Fig1: Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.

  • Fig2: ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig4: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.

  • Fig5: Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Product Profile

Product Name Tyrosinase
Antibody Type Primary Antibodies
Product description 
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Immunogen 
Peptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
WB:1:200-1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:200:
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
ATN antibody CMM8 antibody LB24 AB antibody LB24-AB antibody Monophenol monooxygenase antibody OCA1 antibody OCA1A antibody OCAIA antibody Oculocutaneous albinism IA antibody SHEP3 antibody SK29 AB antibody SK29-AB antibody Tumor rejection antigen AB antibody TYR antibody TYRO_HUMAN antibody tyrosinase (oculocutaneous albinism IA) antibody Tyrosinase antibody
Molecular Weight(MW)

70 kDa

Cellular Localization Membrane.

Database Links

SwissProt ID P14679

Application

  • Application

    Fig1: Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control B16F1, Hela, human urethral melanoma tissue, A431.
Application Notes
WB:1:200-1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:200:

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM638963
  • Price: $336.00
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  • Lead time: 4~6 Week

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