A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (Cat. #AJ1192c), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.
B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAb (Cat. #AJ1192c).
MYC Myc proto-oncogene protein c-Myc Transcription factor p64
Molecular Weight(MW)
48804 Da
Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes
Tissue Specificity
A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology.
A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (Cat. #AJ1192c), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.
Application
B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAb (Cat. #AJ1192c).
Application Notes
WB~~1:1000:
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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