Rabbit Polyclonal antibody to Myc (Phospho Ser373)Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Immunogen
Peptide sequence around phosphorylation site of serine 373 derived from Human Myc.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
IHC-PWB
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
MRTL bHLHe39 c-Myc MYC avian myelocytomatosis viral oncogene homolog myc-related translation/localization regulatory factor class E basic helix-loop-helix protein 39 transcription factor p64 proto-oncogene c-Myc myc proto-oncogene protein v-myc
Target
Myc (Phospho Ser373)
Molecular Weight(MW)
60 (note)
Application
Application
Western blot analysis of extracts from A431 cells untreated or treated with EGF using Myc (Phospho-Ser373) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Ser373) Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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