ICC/IF analysis of methanol-fixed HeLa cells using c-Myc (phospho Thr358) antibody (red) and DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr358) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HT29 cells untreated (lane 1) or treated with UV (lane 2) using Myc (Phospho-Thr358) Antibody
Rabbit Polyclonal antibody to Myc (Phospho Thr358)Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Immunogen
Peptide sequence around phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from Human Myc.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ICC/IFIHC-PWB
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
MRTL bHLHe39 c-Myc MYC avian myelocytomatosis viral oncogene homolog myc-related translation/localization regulatory factor class E basic helix-loop-helix protein 39 transcription factor p64 proto-oncogene c-Myc myc proto-oncogene protein v-myc
Target
Myc (Phospho Thr358)
Molecular Weight(MW)
60 (note)
Application
Application
ICC/IF analysis of methanol-fixed HeLa cells using c-Myc (phospho Thr358) antibody (red) and DAPI (blue).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Phospho-Thr358) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 cells untreated (lane 1) or treated with UV (lane 2) using Myc (Phospho-Thr358) Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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