Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in human heart tissue.
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.
Immunofluorescence: n-Myc Antibody (NMYC-1) - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Mouse monoclonal [NMYC-1] to n-MycMembers of the Myc gene (c-Myc, n-Myc, and L-Myc) family code for proteins that homodimerize but do not bind well to DNA. Myc-Max heterodimers, however, bind with great affinity to the sequence CACGTG and act to initiate transcription of target genes.
Immunogen
Synthetic peptide: SPYVESEDAPPQKC conjugated to KLH, corresponding to amino acids 327-339 of Human n-Myc.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
NMYC-1
Host Species
Mouse
Tested Applications
GSAICC/IFIPWBChIP
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:1000.
For ChIP: Use at a dilution of 1:10-1:500.
For GS: Use at a dilution of 1:1-1:100.
For ICC/IF: Use at a dilution of 1:50-1:100.
For IP: Use at a dilution of 1:10-1:100.
Optimal dilutions should be determined experimentally by the researcher.:
Species Reactivity
HumanMouse
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration)
Purification
Protein A
Target Information
Gene Synonyms
P04198 nMyc ODED MYCN 164280 MODED 4613 N-myc n Myc
Target
n-Myc
Tissue Specificity
This antibody does not react with c-Myc or L-Myc.
Light Chain Type
kappa
Application
Application
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in human heart tissue.
Application
Western Blot: n-Myc Antibody (NMYC-1) - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.
Application
Immunofluorescence: n-Myc Antibody (NMYC-1) - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Application Notes
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:1000.
For ChIP: Use at a dilution of 1:10-1:500.
For GS: Use at a dilution of 1:1-1:100.
For ICC/IF: Use at a dilution of 1:50-1:100.
For IP: Use at a dilution of 1:10-1:100.
Optimal dilutions should be determined experimentally by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer
Tris-glycine, 150 mM NaCl and 0.05% Sodium Azide as preservative.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.