Immunohistochemical
Mouse adult cortexPrimary Antibody Dilution :1:1000Secondary Antibody :Donkey anti rabbit IgG Alexa 594Secondary Antibody Dilution :1:1000Gene Name :POU3F2 Submitted by :Dr. Jin Hee Lee, Ph.D., Winthrop-University HospitalImmunohistochemical
Primary Metastatic Melenoma SpecimanPrimary antibody dilution: 1:1000Western blot
0.5ug/mlELISA Titer: 1:12500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-POU3F2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRN2, OCT7, OTF7, POUF3, OTF-7, brn-2, oct-7 |
| Immunogen | The immunogen for anti-POU3F2 antibody: synthetic peptide directed towards the middle region of human POU3F2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovinePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU3F2 |
|---|---|
| Gene Synonyms | BRN2 OCT7 OTF7 POUF3 OTF-7 brn-2 oct-7 |
| Gene Full Name | POU class 3 homeobox 2 |
| Gene Summary | N-Oct-3 (POU3F2) is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.N-Oct-3 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176), and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.[supplied by OMIM]. |
| Alternative Names | BRN2 OCT7 OTF7 POUF3 OTF-7 brn-2 oct-7 |
| Molecular Weight(MW) | 47kDa |
| Sequence | 443 amino acids |
Immunohistochemical
Mouse adult cortexPrimary Antibody Dilution :1:1000Secondary Antibody :Donkey anti rabbit IgG Alexa 594Secondary Antibody Dilution :1:1000Gene Name :POU3F2 Submitted by :Dr. Jin Hee Lee, Ph.D., Winthrop-University Hospital
Immunohistochemical
Primary Metastatic Melenoma SpecimanPrimary antibody dilution: 1:1000
Western blot
0.5ug/mlELISA Titer: 1:12500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
