Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | POU3F2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POU3F2, BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7, N-Oct3 |
| Product description | N-Oct-3 (POU3F2) is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. N-Oct-3 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176), and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.[supplied by OMIM].1) Goodall, J., (2004) Mol. Cell. Biol. 24 (7), 2923-2931. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU3F2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| POU3F2 antibody can be used for detection of POU3F2 by ELISA at 1:62500. POU3F2 antibody can be used for detection of POU3F2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU3F2 |
|---|---|
| Alternative Names | POU3F2 BRN2 OCT7 OTF7 OTF-7 POUF3 brn-2 oct-7 N-Oct3 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | POU3F2 antibody can be used for detection of POU3F2 by ELISA at 1:62500. POU3F2 antibody can be used for detection of POU3F2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU3F2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
