Application
Immunofluorescence of POU3F2 in human brain tissue with POU3F2 antibody at 20 μg/mL.Application
Western blot analysis of POU3F2 in 3T3 cell lysate with POU3F2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | POU3F2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POU3F2 Antibody: BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7, N-Oct3, BRN2, POU domain, class 3, transcription factor 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Brain-2 |
| Product description | POU3F2 Antibody: POU3F2, also commonly known as brain-2, is a member of a family of POU domain genes expressed in mouse brain and is thought to be involved in the development of the neocortex and establishment of neural cell lineage. Recent studies suggest that POU3F2 may be involved with the development of neurodegenerative diseases as well as tumor development and proliferation. Along with the neural-lineage-specific transcription factors ASCL1 and MYT1L, POU3F2 can convert fibroblasts to functional neurons in vitro, a form of artificial stem cells termed induced neuronal (iN) cells, suggesting that these cells may be useful in the treatment of neurodegenerative diseases.1) Hara Y, Rovescalli AC, Kim Y, et al. Structure and evolution of four POU domain genes expressed in mouse brain. Proc. Natl. Acad. Sci. USA 1992; 89:3280-4. |
| Immunogen | Rabbit polyclonal POU3F2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human POU3F2. The immunogen is located within the last 50 amino acids of POU3F2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU3F2 |
|---|---|
| Alternative Names | POU3F2 Antibody: BRN2 OCT7 OTF7 OTF-7 POUF3 brn-2 oct-7 N-Oct3 BRN2 POU domain class 3 transcription factor 2 Nervous system-specific octamer-binding transcription factor N-Oct-3 Brain-2 |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family. |
Application
Immunofluorescence of POU3F2 in human brain tissue with POU3F2 antibody at 20 μg/mL.
Application
Western blot analysis of POU3F2 in 3T3 cell lysate with POU3F2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
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| Application Notes | POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | POU3F2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | POU3F2 Antibody is supplied in PBS containing 0.02% sodium azide. |
