Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-POU1F1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIT1, CPHD1, GHF-1, Pit-1, POU1F1a |
| Immunogen | The immunogen for anti-POU1F1 antibody: synthetic peptide directed towards the C terminal of human POU1F1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGoatHumanMousePigRabbitRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU1F1 |
|---|---|
| Gene Synonyms | PIT1 CPHD1 GHF-1 Pit-1 POU1F1a |
| Gene Full Name | POU class 1 homeobox 1 |
| Gene Summary | PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP. |
| Alternative Names | PIT1 CPHD1 GHF-1 Pit-1 POU1F1a |
| Molecular Weight(MW) | 33kDa |
| Sequence | 291 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
