Application
Antibody used in IHC on Human Liver cell lysates at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | POU1F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.1) Dattani, M.T., et al., (2003) GrowthHorm.IGFRes.16(9), 1207-1209. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU1F1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| POU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:12500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1.0-10.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU1F1 |
|---|---|
| Alternative Names | POU1F1 PIT1 CPHD1 GHF-1 Pit-1 POU1F1a |
| Molecular Weight(MW) | 33 kDa |
Application
Antibody used in IHC on Human Liver cell lysates at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | POU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:12500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1.0-10.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU1F1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
