Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Live| Product Name | Rabbit anti-POU1F1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GHF-1, PIT1, Pit-1, Pit-1 beta, CPHD1, POU1F1a |
| Immunogen | The immunogen for anti-POU1F1 antibody: synthetic peptide directed towards the middle region of human POU1F1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGoatHumanMousePigRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POU1F1 |
|---|---|
| Gene Synonyms | GHF-1 PIT1 Pit-1 Pit-1 beta CPHD1 POU1F1a |
| Gene Full Name | POU class 1 homeobox 1 |
| Gene Summary | PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP. This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | GHF-1 PIT1 Pit-1 Pit-1 beta CPHD1 POU1F1a |
| Molecular Weight(MW) | 33kDa |
| Sequence | 291 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
