Immunohistochemistry
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-NR0B1 antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Product Name | Rabbit anti-NR0B1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2 |
| Immunogen | The immunogen for anti-NR0B1 antibody: synthetic peptide directed towards the middle region of human NR0B1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR0B1 |
|---|---|
| Gene Synonyms | AHC AHCH AHX DAX-1 DAX1 DSS GTD HHG NROB1 SRXY2 |
| Gene Full Name | Nuclear receptor subfamily 0, group B, member 1 |
| Gene Summary | NR0B1 is a protein that contains a DNA-binding domain. The protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in its gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | AHC AHCH AHX DAX-1 DAX1 DSS GTD HHG NROB1 SRXY2 |
| Molecular Weight(MW) | 52kDa |
| Sequence | 470 amino acids |
Immunohistochemistry
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-NR0B1 antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
