Western blot analysis of NROB1 using rabbit polyclonal NROB1 Antibody (Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NROB1 gene (Lane 2).
NROB1contains a DNA-binding domain and acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in the NROB1 gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.1) Calliari,L.E., Genet. Mol. Res.(2), 177-183 (2007)
Immunogen
This DAX1 (NR0B1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the C-terminal region of human DAX1 (NR0B1).
Western blot analysis of NROB1 using rabbit polyclonal NROB1 Antibody (Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NROB1 gene (Lane 2).
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.