Application
NR0B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Western blot analysis of NR0B1 using rabbit polyclonal NR0B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NR0B1 gene.| Product Name | NR0B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, NR0B1, AHC, DAX1 |
| Product description | This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.1) Li, N., et al. J. Clin. Endocrinol. Metab. 95 (9), E104-E111 (2010) : |
| Immunogen | This NR0B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the Central region of human NR0B1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR0B1 |
|---|---|
| Alternative Names | Nuclear receptor subfamily 0 group B member 1 DSS-AHC critical region on the X chromosome protein 1 Nuclear receptor DAX-1 NR0B1 AHC DAX1 |
| Molecular Weight(MW) | 52 kDa |
Application
NR0B1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Western blot analysis of NR0B1 using rabbit polyclonal NR0B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NR0B1 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
