Application
Antibody used in IHC on Human Testis lysate at 5.0 ug/ml.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | NR0B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR0B1, AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2 |
| Product description | NR0B1 is a protein that contains a DNA-binding domain. The protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in its gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Kim, G.S., (2008) Biochem. Biophys. Res. Commun. 370 (2), 264-268. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR0B1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| NR0B1 antibody can be used for detection of NR0B1 by ELISA at 1:312500. NR0B1 antibody can be used for detection of NR0B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR0B1 |
|---|---|
| Alternative Names | NR0B1 AHC AHCH AHX DAX-1 DAX1 DSS GTD HHG NROB1 SRXY2 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in IHC on Human Testis lysate at 5.0 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | NR0B1 antibody can be used for detection of NR0B1 by ELISA at 1:312500. NR0B1 antibody can be used for detection of NR0B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR0B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
