Immunohistochemical
Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStopPrimary Antibody Dilution :1:50Secondary Antibody :Goat anti-rabbit-cy3Secondary Antibody Dilution :1:1500Color/Signal Descriptions :GLI2: Green Neural crest cells:: RedGene Name :GLI2Submitted by :AnonymousWestern blot
Host: Rabbit Target Name:GLI2 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 4.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 6%-18%Western blot
Host: Rabbit Target Name: GLI2 Sample Tissue: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-GLI2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HPE9, THP1, THP2 |
| Immunogen | The immunogen for anti-GLI2 antibody: synthetic peptide directed towards the N terminal of human GLI2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GLI2 |
|---|---|
| Gene Synonyms | HPE9 THP1 THP2 |
| Gene Full Name | GLI family zinc finger 2 |
| Gene Summary | GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. |
| Alternative Names | HPE9 THP1 THP2 |
| Molecular Weight(MW) | 133kDa |
| Sequence | 1258 amino acids |
Immunohistochemical
Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStopPrimary Antibody Dilution :1:50Secondary Antibody :Goat anti-rabbit-cy3Secondary Antibody Dilution :1:1500Color/Signal Descriptions :GLI2: Green Neural crest cells:: RedGene Name :GLI2Submitted by :Anonymous
Western blot
Host: Rabbit Target Name:GLI2 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 4.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 6%-18%
Western blot
Host: Rabbit Target Name: GLI2 Sample Tissue: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
