Immunohistochemical
ImmunohistochemicalWestern blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-RNF12 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF12, NY-REN-43 |
| Immunogen | The immunogen for anti-RNF12 antibody: synthetic peptide directed towards the C terminal of human RNF12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF12 |
|---|---|
| Gene Synonyms | RNF12 NY-REN-43 |
| Gene Full Name | Ring finger protein, LIM domain interacting |
| Gene Summary | RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported. |
| Alternative Names | RNF12 NY-REN-43 |
| Molecular Weight(MW) | 69kDa |
| Sequence | 624 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
