Application
RLIM Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RLIM antibody detected the RLIM protein (arrow).| Product Name | RLIM Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF12 |
|---|---|
| Alternative Names | RLIM RNF12 E3 ubiquitin-protein ligase RLIM LIM domain-interacting RING finger protein RING finger LIM domain-binding protein RING finger protein 12 Renal carcinoma antigen NY-REN-43 |
| Molecular Weight(MW) | 68549 Da |
| Function | E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex- related dosage differences of X-encoded genes in somatic cells of female placental mammals |
| Tissue Specificity | Expressed in many tissues. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 51132 |
|---|---|
| Nucleotide Accession | NP_057204.2 |
Application
RLIM Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RLIM antibody detected the RLIM protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
