Immunohistochemical
ImmunohistochemicalWestern blot
2.0-4.0ug/mlPositive Control: Daudi cell lysateWestern blot
Lane 1: 20ug untransfected H1299 cells Lane 2: 20ug siRUVBL2 transfected H1299 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:RUVBL2 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:| Product Name | Rabbit anti-RUVBL2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RVB2, TIH2, ECP51, TIP48, CGI-46, INO80J, REPTIN, TIP49B |
| Immunogen | The immunogen for anti-RUVBL2 antibody: synthetic peptide directed towards the N terminal of human RUVBL2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RUVBL2 |
|---|---|
| Gene Synonyms | RVB2 TIH2 ECP51 TIP48 CGI-46 INO80J REPTIN TIP49B |
| Gene Full Name | RuvB-like 2 (E. coli) |
| Gene Summary | RuvB-Like 2 (48-kDa TATA box-binding protein-interacting protein, Reptin 52, RUVBL2) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this protein has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. |
| Alternative Names | RVB2 TIH2 ECP51 TIP48 CGI-46 INO80J REPTIN TIP49B |
| Molecular Weight(MW) | 51kDa |
| Sequence | 463 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
2.0-4.0ug/mlPositive Control: Daudi cell lysate
Western blot
Lane 1: 20ug untransfected H1299 cells Lane 2: 20ug siRUVBL2 transfected H1299 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:RUVBL2 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
