RUVBL2 encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities.1) Bauer, A., et al., (2000) EMBO J. 19 (22), 6121-6130.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUVBL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
RUVBL2 antibody can be used for detection of RUVBL2 by ELISA at 1:1562500. RUVBL2 antibody can be used for detection of RUVBL2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on K562 at 1:200.
Application
Antibody used in IHC on Human Liver.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
RUVBL2 antibody can be used for detection of RUVBL2 by ELISA at 1:1562500. RUVBL2 antibody can be used for detection of RUVBL2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RUVBL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.