Immunohistochemical
ImmunohistochemicalWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Daudi cell lysate| Product Name | Rabbit anti-TAF1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BA2R, CCG1, CCGS, DYT3, KAT4, NSCL2, OF, P250, TAF2A, TAFII250, XDP, N-TAF1, DYT3/TAF1 |
| Immunogen | The immunogen for anti-TAF1 antibody: synthetic peptide directed towards the C terminal of human TAF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCChIP |
| WB:1:500~1:2000 IHC:1:50~1:200 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF1 |
|---|---|
| Gene Synonyms | BA2R CCG1 CCGS DYT3 KAT4 NSCL2 OF P250 TAF2A TAFII250 XDP N-TAF1 DYT3/TAF1 |
| Gene Full Name | TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF1 encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene. |
| Alternative Names | BA2R CCG1 CCGS DYT3 KAT4 NSCL2 OF P250 TAF2A TAFII250 XDP N-TAF1 DYT3/TAF1 |
| Molecular Weight(MW) | 215kDa |
| Sequence | 1872 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Daudi cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
