Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.| Product Name | TAF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAF1, BA2R, CCG1, CCGS, DYT3, KAT4, NSCL2, OF, P250, TAF2A, TAFII250, XDP, N-TAF1, DYT3/TAF1, TAFII-250, TAF(II)250 |
| Product description | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF1 encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.1) Herzfeld, T., (2007) Mamm. Genome 18 (11), 787-795. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TAF1 antibody can be used for detection of TAF1 by ELISA at 1:312500. TAF1 antibody can be used for detection of TAF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF1 |
|---|---|
| Alternative Names | TAF1 BA2R CCG1 CCGS DYT3 KAT4 NSCL2 OF P250 TAF2A TAFII250 XDP N-TAF1 DYT3/TAF1 TAFII-250 TAF(II)250 |
| Molecular Weight(MW) | 215 kDa |
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | TAF1 antibody can be used for detection of TAF1 by ELISA at 1:312500. TAF1 antibody can be used for detection of TAF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TAF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
