Immunohistochemical
DG of Hippocampus Dilution: 1:200Immunohistochemistry
Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-NFIX antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-NFIX polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NF1A |
| Immunogen | The immunogen for anti-NFIX antibody: synthetic peptide directed towards the middle region of human NFIX |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFIX |
|---|---|
| Gene Synonyms | NF1A |
| Gene Full Name | Nuclear factor I/X (CCAAT-binding transcription factor) |
| Gene Summary | Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers. |
| Alternative Names | NF1A |
| Molecular Weight(MW) | 49kDa |
| Sequence | 441 amino acids |
Immunohistochemical
DG of Hippocampus Dilution: 1:200
Immunohistochemistry
Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-NFIX antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
