Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on mouse hippocampus at 1:200.Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.| Product Name | NFIX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFIX, NF1A, MRSHSS, SOTOS2 |
| Product description | Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.1) Norquay, L.D., et al., (2003) Mol. Endocrinol. 17 (6), 1027-1038. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIX. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFIX antibody can be used for detection of NFIX by ELISA at 1:312500. NFIX antibody can be used for detection of NFIX by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFIX |
|---|---|
| Alternative Names | NFIX NF1A MRSHSS SOTOS2 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on mouse hippocampus at 1:200.
Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NFIX antibody can be used for detection of NFIX by ELISA at 1:312500. NFIX antibody can be used for detection of NFIX by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFIX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
