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UBE2N antibody - middle region

Catalog Number:OM106195
OM106195
Discount Price
OM106195-100ul
1~2 Week
100ul
OM106195-50ul
50ul
OM106195-20ul
20ul
1~2 Week
Product Profile
Product Name UBE2N antibody - middle region
Antibody Type Primary Antibodies
Immunogen The immunogen for anti-UBE2N antibody: synthetic peptide directed towards the middle region of human UBE2N
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WB,IHC
Species Reactivity Bovine,Guinea Pig,Human,Mouse,Rabbit,Rat,Sheep,Zebra Fish
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol UBE2N
Gene Synonyms MGC131857
MGC8489
UBC13
UbcH-ben
Gene Full Name Ubiquitin-conjugating enzyme E2N
Gene Summary UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Alternative Names MGC131857
MGC8489
UBC13
UbcH-ben
Molecular Weight(MW) 17kDa
Sequence 152 amino acids
Database Links
Entrez Gene 7334
SwissProt ID P61088
Protein Accession NP_003339
Application

Immunohistochemical

Mouse brain stem cells
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:1000
Color/Signal Descriptions :
UBE2N: Red DAPI:Blue
Gene Name :
UBE2N
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Immunoblot

1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein.
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:50,000
Gene Name:
UBE2N
Submitted by:
Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.

Western blot

Sample Type: 1. Human NT-2 cells (60ug)
2. mouse brain extracts (80ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science 

Western blot

2.5ug/ml
Positive Control: Jurkat cell lysate
Additional Information
Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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