Immunohistochemical
Mouse brain stem cellsPrimary Antibody Dilution :
1:500Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :
1:1000Color/Signal Descriptions :
UBE2N: Red DAPI:BlueGene Name :
UBE2NSubmitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunoblot
1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein.
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:50,000
Gene Name:
UBE2N
Submitted by:
Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.
Western blot
Sample Type: 1. Human NT-2 cells (60ug)
2. mouse brain extracts (80ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science
Western blot
2.5ug/ml
Positive Control: Jurkat cell lysate