Application
Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.Application
Immunofluorescence of UBC13 in Mouse Thymus cells with UBC13 antibody at 10 μg/mL.Application
Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2.5 μg/ml.Application
Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2 μg/mL.| Product Name | UBC13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBC13 Antibody: UBC13, UbcH13, HEL-S-71, UbcH-ben, BLU, Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme |
| Product description | UBC13 Antibody: Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway. It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.1) Brusky J, Zhu Y, and Xiao W. UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. Curr. Genet. 2000; 37:168-74. |
| Immunogen | UBC13 antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13. The immunogen is located within the last 50 amino acids of UBC13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| UBC13 antibody can be used for detection of UBC13 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2N |
|---|---|
| Alternative Names | UBC13 Antibody: UBC13 UbcH13 HEL-S-71 UbcH-ben BLU Ubiquitin-conjugating enzyme E2 N Bendless-like ubiquitin-conjugating enzyme |
| Sequence Similarities | 2) Cat. No. 1409 - Mouse Thymus Tissue Lysate |
Application
Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Application
Immunofluorescence of UBC13 in Mouse Thymus cells with UBC13 antibody at 10 μg/mL.
Application
Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2.5 μg/ml.
Application
Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | UBC13 antibody can be used for detection of UBC13 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UBC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | UBC13 Antibody is supplied in PBS containing 0.02% sodium azide. |
