Application
Antibody used in WB on r-UBE2N at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).Application
Antibody used in IHC on Human brainstem at 1:500.Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.| Product Name | UBE2N Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2N, UBC13, UbcH13, HEL-S-71, UbcH-ben |
| Product description | UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.1) Bothos, J., et al., (2003) Oncogene 22 (46), 7101-7107. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2N. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UBE2N antibody can be used for detection of UBE2N by ELISA at 1:312500. UBE2N antibody can be used for detection of UBE2N by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogDrosophilaHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UBE2N |
|---|---|
| Alternative Names | UBE2N UBC13 UbcH13 HEL-S-71 UbcH-ben |
| Molecular Weight(MW) | 17 kDa |
Application
Antibody used in WB on r-UBE2N at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).
Application
Antibody used in IHC on Human brainstem at 1:500.
Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | UBE2N antibody can be used for detection of UBE2N by ELISA at 1:312500. UBE2N antibody can be used for detection of UBE2N by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2N antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
